منابع مشابه
Two-dimensional Electrophoresis
Two-dimensional (2D) polyacrylamide gel electrophoresis is a classical technique for the separation of proteins. It Rrst appeared in the mid-1970s but for a long time it only found limited applications. Recently it has enjoyed an impressive renaissance. The major reasons for this are the introduction of the immobilized pH gradient (IPG) strips and the development of analytical methods capable o...
متن کاملTwo-Dimensional Electrophoresis
We compared cellular proteins from normal human blood lymphocytes, monocytes, and granulocytes, using highresolution two-dimensional electrophoresis. The leukocytes were isolated from peripheral blood by centrifugation on density step gradients (yielding a fraction of purified granulocytes and a lymphocyte/monocyte mixture) and monocytes were subsequently separated from lymphocytes by virtue of...
متن کاملProtein extraction and comparison of stain protocols for analysis of two-dimensional electrophoresis gels.
Protein extraction and the proteome of Lactobacillus delbrueckii subsp. bulgaricus were studied using different stains. The reversible silver staining technique was shown to be more sensitive than the irreversible silver stain. Coomassie colloidal was demonstrated to be as sensitive as reversible silver stain; however, the Coomassie colloidal blue solution developed a higher background and for ...
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ژورنال
عنوان ژورنال: European Journal of Histochemistry
سال: 2010
ISSN: 2038-8306,1121-760X
DOI: 10.4081/ejh.2010.e29